Lokhu kufinyelelwe ngokufakwa kwe-alcohols kanye noketshezi olukhethekile olushintsha inkomba yokuthinta izicubu
Ososayensi base-University of Munich benza noma iyiphi ingxenye yomzimba wesilwane ibe sobala ngokuphelele, kubika i RIA Novosti. Lokhu kufinyelelwe ngokufakwa kwe-alcohols noketshezi olukhethekile olushintsha inkomba yokuthinta izicubu.
Ngokusho komunye wababhali balolu cwaningo, u-Chen Pan, "ukushisa" umzimba wesilwane, kudingeka ama-reagents amathathu kuphela - itot-butanol ekhethekile, inhlanganisela ye-benzene ne-alcohol compounds ebizwa nge-BABB, ne-diphenyl ether enezinhlanganisela ezincane ze-Vitamin E.
UPan nozakwabo bakhethe inhlanganisela eminingana ye-ether ne-BABB ingxubevange, eminye yayo "ebunjiwe" ukuvikela izinto ezikhanyayo, nabanye - ukwandisa ukubonakala kwendwangu. "I-Bleaching" kuthatha imizuzu engama-45 kuya emahoreni athile.
Njengobufakazi bempumelelo yalolucwaningo, izazi zebhayoloji “zahlanganisa” amagundane amaningi wonke ngokugcina izicubu zazo zezinzwa ngegciwane le-AAV, elafaka uhlobo lwe-GFP kuma-neurons abo, okubenza bakhazimula luhlaza. Ososayensi baphinde balungiselela amasampula amaningana okugcwala kwengqondo okusobala futhi “okugqanyisiwe” kwamagundane namagundane, kufaka phakathi nentambo yomgogodla exhunywe kubo.
Ngokusho kwababhali balesi sivivinyo, ukukhula kwabo kuzosheshisa ukutadisha izimfihlo zobuchopho nezinye izingxenye eziyinkimbinkimbi zomzimba.
Eminyakeni embalwa edlule, izazi zesayensi yokufunda izinto eziphilayo kanye nezemvelo yamakhemikhali zenze ubuchwepheshe obuningana obwenza sikwazi ukwenza izicubu zobuchopho noma lonke uhlelo lwezinzwa lube sobala, ekuvumela ukuthi ubhekele ukuthi ama-neurons "axhumana" kanjani nomunye nokuthi axhumeke kuphi.
UCan Pan waphawula ukuthi wonke la makhono anezithiyo ezimbili. Zidinga “ukususwa” kobuchopho noma esinye isitho somzimba ngaphambi kokuba senziwe esobala, noma akuvumeli ukusetshenziswa kwamalebula ahlukahlukene we-fluorescent kanye nodayi, ososayensi abethula emzimbeni wesilwane abesifunda ngesikhathi sokuphila kwakhe ukumaka izingxenye zesistimu yezinzwa abazithandayo noma omunye umzimba.
Konke lokhu empeleni kwenza amasu anjena anisho lutho, ngoba lezi zinkinga azivumeli ukutadisha ngokuphelele ukwakheka kwezitho "ezihlanganisiwe", futhi baqonde ukuthi basebenzisana kanjani nezinye izingxenye zomzimba.
UPan nozakwabo bakwazile ukunqoba zombili lezi zinkinga ngokwenza ngcono umsebenzi wenye yale teknoloji - indlela ye-3DISCO, eyakhiwa ngababhali abathile ngonyaka we-2012. Ukuphindeka kwaso nje ukuthi lapho kulungiswa wonke ama-molecule akhanyayo futhi “anemibala” angeniswe emzimbeni abhujiswa ngokungenakuphikiswa.
Indawo yethu inemithetho yokuziphatha esikukhuthaza ukuthi uyilandele. Kumazwana anqatshelwe:
- inhlamba
- ubiza udlame, ukuthukwa ngezizathu zobuhlanga
- ukuthuka ababhali bezinto zokwakha, abanye abasebenzisi besayithi
- ukukhangisa, izixhumanisi kwezinye izinsiza, izingcingo nabanye oxhumana nabo
Abahleli ababheki oxhumana nabo, ngokubheka njenge-priori eyingozi kwabanye abasebenzisi. Imilayezo enalezi zokwephulwa isuswa umengameli. Siphinde sazise ukuthi ibhodi yokuhlela ayizibophezeli kokuqukethwe okuphawulayo, noma ngabe isikhundla sabasebenzisi singahambisani nemibono yebhodi lokuhlela
Izazi zesayensi yezinto eziphilayo zaseJalimane ziveze imfihlo yokungabonakali. Ososayensi bakwazi ukwenza igundane esobala ngokushintsha inkomba yokucima izicubu.
Ososayensi bafunde "ukucwilisa" noma iyiphi ingxenye yomzimba we-rodent, nokubenza babonakale ngokuphelele ngokugcwala kwe-alcohols kanye noketshezi olukhethekile olushintsha inkomba yezicubu ezibucayi, ngokusho kwendatshana ekhishwe ephephabhukwini leNdalo Yezindlela.
“Ukuxubha” kobuchopho noma umzimba wonke wesilwane kungenziwa ngemizuzu engama-45 noma amahora ambalwa, futhi lokhu kuzodinga ama-reagents amathathu kuphela - ikhethelo lotshwala elikhethekile, ingxube ye-benzene ne-alcohol compounds ebizwa nge-BABB, ne-diphenyl ether enezinhlanganisela ezincane zevithamini E ", - kusho ocwaningweni lwezesayensi, kubika i RIA Novosti.
Le ndlela ibizwa nge-3DISCO. Ukuthuthuka kwayo kwaqala ngonyaka ka-2012. Enye yezinto ezidala ukuthi kube nzima ngale ndlela ukuthi ngesikhathi sokulungiselela amalungiselelo wonke ama-molecule akhanyayo abhujiswe ngokungenakuphikiswa. Lokhu kusho ukuthi into ngeke iphinde ibonakale.
Ngokusho kososayensi, izindlela ezinjalo “zokuxubha” izicubu eziphilayo zidingeka kakhulu ukuqonda ukuthi ubuchopho bethu nezinye izingxenye zomzimba zakhiwa kanjani. Ababhali balolu cwaningo banethemba lokuthi ukuthuthuka kwabo kuzosiza ukunciphisa isibalo segundane nezinye izilwane zokuhlola okumele zikhutshazwe ukwenza lezo zivivinyo.
